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Experiment Design
Data File Format
Program Cutoffs
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Please describe your experimental design using following parameters
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in each row for help (in a new window)
The Probe Name (Letters and Numbers ONLY)
Description of the Experiments
Is this a Yeast Kinase Assay?
YES
NO
Number of Slides for One Experiment?
Two
One
Negative control experiemnts
No Neg control
One Neg control
Two Neg control
Annotation Report for the hits
No annotation
Yeast SGD Anno
Human Chip
Whether the signals are positive or negative?
Negative
Positive
The amount of protein on the array
Available
Not Available
Does the Protein Amount File Have Background Column?
YES(Homemade PA data)
NO(Commercial PA QC)
Are Eyeball Positive spots Labelled?
YES
NO
Are proteins printed in duplicates right next to each other?
YES
NO
Remove potential smudges
YES
NO
Background Normalization for smears
Use Back Norm
No
Foreground Normalization for spatial artifacts
Use Fore Norm
No